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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant position in marketing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence data is not however out there.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide commonly contains the epitope identified via the antibody.
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This guide outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible outcomes are...
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This guide outlines further feasible optimization of cellular and immunoassay parameters to make sure the absolute best benefits are...
Antibodies bound to the blocking peptide no more bind towards the epitope around the concentrate on protein. By evaluating the staining within the blocked antibody vs . the antibody alone, one can see which staining is unique.
Target Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which contribute to your temporal coordination of every mitotic party. Cyclin E kinds a posh with and functions being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates on the G1-S phase boundary and is degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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